Replicating Diseased Communities with Modified SHI Media *Number one downloaded paper from Wiley Publishing 2022!

Dec 15

Written By Kristopher Kerns

Developing a laboratory model of oral polymicrobial communities is essential for in vitro studies of the transition from healthy to diseased oral plaque. Here, we systematically modified SHI medium components to investigate the impacts of varying n… — Developing a laboratory model of oral polymicrobial communities is essential for in vitro studies of the transition from healthy to diseased oral plaque. Here, we systematically modified SHI medium components to investigate the impacts of varying nutrients and develop a medium capable of supporting a specific disease-state subgingival community. Biofilms grown in subSHI-v1 medium retained high diversity closest to the source inoculum and maintained greater proportions of many gram-negative species of interest from the subgingival periodontal pocket (including members of the genera Prevotella and Treponema, and the Candidate Phyla Radiation phylum Saccharibacteria)
Lamont, E.I., Gadkari, A., Kerns, K.A., To, T.T., Daubert, D., Kotsakis, G., Bor, B., He, X., McLean, J.S. Modified SHI medium supports growth of a disease-state subgingival polymicrobial community in vitro. Molecular Oral Microbiology. 2020 Nov 11. https://doi.org/10.1111/omi.12323 PubMed PMID: 33174294

Kristopher Kerns

UW researchers reveal three inflammatory clinical responder types to subgingival microbial growth in humans (PNAS) *Received the AAP Clinical Research Award 2022

Our work on nano-sized parasitic bacteria covered in TWiM episode 222